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Abraxis ELISA Assays

Posted by anna on April 13, 2022

Abraxis ELISA assays are used for the detection of endocrine-disrupting chemicals, including hormones and pesticides. The Abraxis ELISA Multi-Screen assay detects Monensin, Maduramicin, and Salinomycin. ELISA assays are also available for a variety of other uses. These assays can be used for a variety of regulatory monitoring applications.

The Abraxis ELISA test kit detects brevetoxins in marine mammals, birds, and sea turtles. The test kit is also effective in detecting specific congeners of brevetoxin. For example, Abraxis ELISA PSP tests can detect the presence of saxitoxins in shellfish. Using this test kit, veterinarians can identify whether a consumer is at risk for this dangerous condition.

Glyphosate is a widely-used herbicide that is widely applied in agricultural areas. Its use coincided with the development of genetically modified crops that tolerate the herbicide. In turn, this allows farmers to selectively target the weeds while protecting important crops. Recently, however, consumer pressure has prompted increased commodity testing of food-borne glyphosate levels. Although countries have set glyphosate limits for food products, the Abraxis Glyphosate ELISA kit has been developed to detect glyphosate at concentrations below these levels.

The Abraxis ELISA kit is compatible with a wide range of microplate readers, including Molecular Devices' VersaMax and Biotek's MicroPlateReader. The Abraxis melamine ELISA kit is available with an industry-standard analysis software, which is required for FDA 21 CFR Part 11 compliance. Moreover, SoftMax Pro GxP Software includes a pre-configured protocol for the Abraxis Melamine ELISA kit.

Aflatoxin ELISA Kit

Aflatoxin ELISA assay kit is suitable for the quantitative analysis of aflatoxin content in foods, feed, and other samples. The method requires no complicated instrumentation, and provides sensitive results quickly. In 45 minutes, you can test 42 samples. For a successful test, sample preparation must be performed. The samples must be extracted using methanol/water before preparing the sample for ELISA.

For direct ELISA, acetone, methanol, or methanol can be used. However, these solvents can decrease the ELISA colour development. Only 40% of the sample should be extracted using acetone. The remaining sample should be centrifuged for 30 seconds. In both cases, the aliquot of the sample must be weighed accurately. If the sample does not contain any aflatoxin, the results should be interpreted in accordance with the kit's instruction manual.

The direct competitive rapid ELISA is highly specific for AFB1 and showed low cross-reactivity with other aflatoxins or metabolites. Methanol, the extraction solvent, was also found to be satisfactory in achieving a high recovery. The ELISA was validated using triplicate samples. The test was reliable, and it showed a high level of aflatoxin B1 contamination. In addition, the ELISA is tolerant to methanol up to 60%. The results of this study show that aflatoxin B1 is well present in feed supplements.

The accuracy of the ELISA was confirmed by HPLC analysis. The test kit was validated using both imported and local corn and feed samples. Both HPLC and ELISA gave similar results, though the aqueous method showed slightly lower average recovery for the metabolites. This result may indicate that the ELISA is insensitive to aflatoxin, but the ELISA gave a better overall result. In order to reduce the errors caused by the residues in the subsequent detection process, remember to clean the plate by using an ELISA washer.

The commercial aflatoxin ELISA kit is an economical and rapid method for detecting aflatoxin. Compared with the chromatographic method, aflatoxin ELISA is cheaper, but it is not always available and still expensive for the Indonesian economy. Therefore, this paper describes the development of a direct competitive ELISA assay for aflatoxin. Such a competitive ELISA can be used to detect aflatoxin in feeds and provide fast management decisions, which may improve the quality and safety of animal feed.

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